labeling technique meaning in English
标记原子
Examples
- By using the gfp - labeling technique , we have found that gfp - cam colocalized with all the characterized structures formed during cytokinesis
通过采用gfp标记技术，我们观察到gfp - cam与胞质分裂期细胞内形成的特征结构存在共分布。 - But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area " . to answer these questions , we observed systemically the synaptic connections among the pv - like immunoreactive neurons , fibers and terminals and the connections between dnpi - like , gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry , triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique
但是目前对新发现的囊泡膜mu转运体一dnn样阳性终末与带状区内pag样阳性神经元之间ej关系，以及谷氨酸脱发酶（ gad ，是gaba能神经元和终末的特异性标识物）是否参与其调控作用，尚缺乏系统的形态学资料。 - The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation . 2 . following lps or seb was administered intraperitoneally , the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice . double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔内给予细菌内毒素lps或肠毒素seb ，用免疫组织化学方法观察了脾脏核增殖抗体及下丘脑室旁核和视上核中1型il 1受体的表达，并采用双标记技术观察了1型il刁受体阳性神经元和加压素及催产素表达的关系。 - The amplification system was optimized so that the pcr with different primers can be carried out under the same condition . the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques . sequences were analyzed and compared base on sequencing analysis3 . 4 and seq / ede software
方法根据mtdna控制区及其周围区域的序列，设计多对引物，探索优化扩增体系，使扩增条件能够同时满足多对引物的需要，用sanger末端终止法及荧光标记技术对样本进行dna测序， sequencinganalysis3 . 4和seq ede软件进行序列分析和比对。 - The characteristics of this method are : a , directly counting cell number without the influence of the metabolic state of the cells ; b , discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c , less treatment step , and free - radioactivity ; d , high sensitivity and reliability . 2 , using the above assay , immunofluorescent labeled technique , and flow cytometry , the pbmc proliferation , apoptosis , necrosis , cell cycle , activation , cytokines and membrane marker were detected . the results showed that the number of pbmc reduced , but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle , but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
结合以上创建的方法和免疫荧光流式细胞术，用k562细胞株可溶性分泌物(上清)对外周血单个核细胞( pbmc )进行培养以模拟体内微环境，然后分别从细胞增殖、凋亡、坏死、细胞周期、活性、细胞因子和表面抗原表达等方面进行研究，结果发现用肿瘤上清培养的pbmc细胞数量下降明显，但同时对其有激活作用，且呈剂量依赖性；细胞数的下降主要是由细胞坏死和凋亡引起的，肿瘤上清对细胞周期没有阻断作用，反而略有促进作用； t细胞亚群比例增加，并促进表达th1 、 th2细胞因子。